The separation of the phosphodiesterase and deoxyribonuclease II activities of bovine spleen.

Inhibition of deoxyribonucleases by phosphorothioate groups in oligodeoxyribonucleotides.

The Rp- and Sp-diastereomers of the phosphorothioate-containing oligonucleotide d[ApAp(S)ApA] have been synthesized. They and the tetramer d[ApApApA] were tested as substrates for staphylococcal nuclease, DNase II and spleen phosphodiesterase. For digestions with DNase I these oligonucleotides were converted to the 5'-phosphorylated derivates. The reactions with the nucleases were analysed by H...

lsolation of parapox viruses from man and animals: cultivation and cellular changes in bovine fetal spleen cells

The chromatographic separation and characterization of the acid and alkaline ribonucleases of bovine spleen and liver.

The occurrence of both acid and alkaline ribonuclease activities in liver and kidney was reported in 1954 by Roth (1) and by de Lamirande et al. (2). Since then, investigators have used various methods to purify the intracellular ribonuclease activities (3-8). In our studies, comparatively mild procedures were used for the nuclease preparations (5)) with the hope that the nuclease activities as...

Comparison of diagnostic tests for the detection of bovine brucellosis in the natural cases of abortion

Rapid and precise diagnosis in natural field cases of bovine abortion caused by Brucella abortus warrants the use of the most sensitive and reliable diagnostic methods. In the present study, bacterial isolation, serology, gross, histopathology, immunohistochemistry and polymerase chain reaction technique(s) were applied. Sero-prevalence studies showed the rate of 28.86% positive cases using the...

The effect of bovine viral diarrhea virus on bovine monocyte phenotype

Bovine viral diarrhea virus (BVDV) is an economically important pathogen of the livestock industry worldwide. BVDV is classified into cytopathic (cp) and noncytopathic (ncp), depending on its effects on cultured cells. BVDV is known to alter the host’s immune response. Of this, major histocompatibility complex (MHC) class II molecules play a central role in the development and function of the i...